Annexin v labeled with alexa fluor 488 in frozen rat placenta section by ihc immunohistochemistry.
Immunofluorescence protocol for frozen sections.
Immunofluorescence on frozen sections.
Brigitte arduini version 1 2015 mar 23.
Immunofluorescence can also be used as a qualitative measure of protein expression.
See cryoprotection and processing of embryonic tissue protocol.
Microscope slides pre coated.
Please refer to the applications section on the front page of product datasheet or product webpage to determine if this product is validated and approved for use on cultured cell lines if ic paraffin embedded samples if p or frozen tissue sections if f.
Immunohistochemistry protocol for frozen sections.
The suggested cryostat temperature is between 15 and 23 c.
Immunofluorescence is commonly used to determine the cellular or tissue localization of a protein of interest.
Store frozen blocks at 80 ºc.
Nagy gertsenstein vintersten and behringer ed.
Cut cryostat sections at 5 10 µm and mount on gelatin coated histological slides.
Immunofluorescence on frozen tissue sections bio protocol.
Immunofluorescence general protocol important.
Protocol for immunofluorescent staining of mouse frozen sections tissue.
Embed the tissue completely in oct compound prior to cryostat sectioning.
Snap frozen fresh tissues in liquid nitrogen or isopentane pre cooled in liquid nitrogen embedded in oct compound in cryomolds.
Icc and if video protocol.
Tissue preparation cyropreservation.
The following is a general procedure guide for preparation and staining of acetone fixed frozen tissues using a purified unconjugated primary antibody biotinylated secondary antibody and streptavidin horseradish peroxidase sav hrp and dab detection system.
This portion of the protocol can be skipped if you are working with pre mounted tissue slides.
Direct vs indirect if.
Cut 4 8 um thick cryostat sections and mount on superfrost plus slides or.
Materials phosphate buffered saline pbs 1x paraformaldehyde pfa 4 see support protocol 1.
Modified from manipulating the mouse embryo 3.
Slides can be safely stored for 6 12 months at 80 c until ready for staining.
Do not allow frozen tissue to thaw before cutting.
Carry out incubations in a humidified chamber to avoid tissue drying out which will lead to non specific binding and high background staining.
Immunocytochemistry and immunofluorescence protocol related fluorescence.
This protocol is also suitable for 40µm free floating.
Immunofluorescence staining protocol.
The section will curl if the specimen is too cold.
Paraffin and frozen sections reagents can be applied manually by pipette or this protocol can be adapted for automated and semi automated systems if these are available.
